Position Statement on Research with Live Bsal Cultures
Due to the potential negative effects of Batrachochytrium salamandrivorans (Bsal) on populations of susceptible host species and the need for cooperative research to maximize the pace of knowledge discovery, the following standard operating procedures were agreed upon by the Bsal Task Force in the USA. To ensure the locations of live Bsal cultures used for research are known and because Bsal has not been isolated in North America, the Bsal Task Force states that all live Bsal cultures used for research must be obtained directly from Drs. Frank Pasmans or An Martel at the University of Ghent, Belgium, using a traditional Material Transfer Agreement. As required, all necessary import and export permits must be obtained. Furthermore, to ensure that Bsal remains contained in research facilities outside of its endemic regions, the pathogen must be handled by trained professionals, and research protocols must adhere to at least Biosafety Level 2 criteria. Following completion of research, live Bsal organisms should be destroyed by heat treatment or by immersion in >10% bleach solution.